منابع مشابه
Picrosirius red staining of cardiac muscle following phosphomolybdic acid treatment.
When the picrosirius red technique was applied to cardiac muscle sections, intense yellow myocyte staining sometimes obscured thin collagenous septa. The picrosirius red technique was modified to include treatment of the sections in 0.2% (w/v) aqueous phosphomolybdic acid prior to staining. With 1-5 min treatment, cytoplasmic staining was eradicated; diminution of collagen staining occurred onl...
متن کاملClinicopathologic study of a series of giant cell fibroma using picrosirius red polarizing microscopy technique.
BACKGROUND Giant cell fibroma (GCF) is a distinct type of fibroma with characteristic large, stellate mononuclear or multinucleated giant fibroblasts; the stroma of GCF is relatively unexplored. The Picrosirus red polarizing microscopy technique is used to characterize the collagen fibers. The aim of this study was to evaluate the staining properties of collagen fibers in GCF and to correlate i...
متن کاملCollagen and Picrosirius Red Staining: a Polarized Light Assessment of Fibrillar Hue and Spatial Distribution
Collagen plays a vital role in maintaining structural integrity and in determining tissue function. Therefore, methods to detect, quantify, and analyze collagen are valuable. Nevertheless, stains historically employed to detect collagen have disadvantages, principally a poor specificity for thin fibers. Conversely, picrosirius red, which has the capability to detect thin fibers, although freque...
متن کاملHistochemical analysis of polarizing colors of collagen using Picrosirius Red staining in oral submucous fibrosis.
BACKGROUND Oral Submucous fibrosis (OSMF) is a common potentially malignant disease, which is characterized by generalized fibrosis of the oral soft tissues. It is due to disproportion in the collagen deposition and degradation. The excess deposition of collagen results in juxta epithelial fibro elastic changes and epithelial atrophy. Neoplastic changes in the epithelium usually precede the cha...
متن کاملDetermination of collagen content within picrosirius red stained paraffin-embedded tissue sections using fluorescence microscopy
Picrosirius red (PSR) staining is a commonly used histological technique to visualize collagen in paraffin-embedded tissue sections. PSR stained collagen appears red in light microscopy. However it is largely unknown that PSR stained collagen also shows a red fluorescence, whereas live cells have a distinct green autofluorescence. Both emission patterns can be detected using standard filter set...
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ژورنال
عنوان ژورنال: Indian Journal of Pathology and Oncology
سال: 2020
ISSN: 2394-6784,2394-6792
DOI: 10.18231/j.ijpo.2020.038